Mesenchymal Stem Cell Medium-serum free (MSCM-sf), when used with Mesenchymal Stem Cell Growth Supplement-serum free (MSCGS-sf, Cat. no. 7562), is a chemically defined medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.