The Mesenchymal Stem Cell Adipogenic Differentiation Medium (MADM) has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis study.
By using the Mesenchymal Stem Cell Adipogenic Differentiation Medium, we obtain:
>35% mature adipocytes from human bone marrow
>50% mature adipocytes from rat bone marrow
>60% mature adipocytes from human mesenchymal stem cell isolated from adipose tissue.
Efficiency of adipogenic differentiation depends on the quanlity of the mesenchymal stem cells.
Mesenchymal stem cell Adipogenic Differentiation Medium (MADM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals.
Components
MADM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. no. SC0025), 5 ml of Mesenchymal Stem Cell Adipogenic Differentiation Supplement (MADS, Cat. no. SC7542), 5 ml of penicillin/streptomycin solution (P/S, Cat. no. SC0503).
Note: MADS may be slightly hazy; Haziness does not affect cell culture and differentiation efficiency.
Storage
Store the basal medium at +4oC and the FBS, MADS and P/S solution at -20oC. Protect from light.
Shipping
Basal medium: room temperature.
Supplements: dry ice.
Product Use
MADM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.