Smooth Muscle Cell Medium (SMCM), when used with Smooth Muscle Cell Growth Supplement (SMCGS, Cat #1152) and 10 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal smooth muscle cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal smooth muscle cells in vitro.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Store the basal medium at 4°C, the SMCGS, the FBS and the P/S solution at -20°C. Protect from light.
Basal medium: room temperature. Supplements: dry ice.