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Cell Type: Ready-to-Use Stem Cells
Propagation Requirements: The optimized medium for this cell line is Rat iPSC Culture Medium available from ABM (TM021). This cell line needs to be cultured on inactivated REF feeder cells (T2017). Atmosphere:air: 95%, CO2: 5%; Temperature: 37.0°C.
Subculture Protocol: 1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new feeder cell culture vessel. 6. Incubate cultures at 37°C. Be sure to use inactivated REF feeder cells (T2017).