Recommended Medium: It is recommended to use Neuronal Maintenance Medium (NMM, Cat. no. SC5951) for culturing HPSC-SN in vitro.
Product Use: HPSC-SN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Basbaum, A. I., Bautista, D. M., Scherrer, G., & Julius, D. (2009). Cellular and molecular mechanisms of pain. Cell, 139(2), 267–284.
[2] Marmigère, F., & Ernfors, P. (2007). Specification and connectivity of neuronal subtypes in the sensory lineage. Nature Reviews Neuroscience, 8(2), 114–127.
[3] Chambers, S. M., et al. (2012). Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nature Biotechnology, 30(7), 715–720.
[4] Young, G. T., et al. (2014). Characterizing human stem cell-derived sensory neurons at the single-cell level reveals their ion channel expression and utility in pain research. Molecular Therapy, 22(8), 1530–1543.