Recommended Medium:It is recommended to use Neuronal Maintenance Medium (NMM, Cat. #5951) for culturing HPSC-MN in vitro.
Product Use: HPSC-MN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Jessell, T. M. (2000). Neuronal specification in the spinal cord: inductive signals and transcriptional codes. Nature Reviews Genetics, 1(1), 20–29.
[2] Shirasaki, R., & Pfaff, S. L. (2002). Transcriptional codes and the control of neuronal identity. Annual Review of Neuroscience, 25, 251–281.
[3] Amoroso, M. W., et al. (2013). Accelerated high-yield generation of limb-innervating motor neurons from human stem cells. Journal of Neuroscience, 33(2), 574–586.
[4] Sances, S., et al. (2016). Modeling ALS with motor neurons derived from human induced pluripotent stem cells. Nature Neuroscience, 19(4), 542–553.