Human Pluripotent Stem Cell Derived-Glutamatergic Neurons

Recommended Medium: It is recommended to use Neuronal Maintenance Medium (NMM, Cat. no SC5951) for culturing HPSC-GlutN in vitro. 

Product Use: HPSC-GlutN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping: Dry ice.

References: [1] Fremeau, R. T., Jr., Troyer, M. D., Pahner, I., Nygaard, G. O., Tran, C. H., Reimer, R. J., … Edwards, R. H. (2001). The expression of vesicular glutamate transporters defines two classes of excitatory synapse. Neuron, 31(2), 247–260.

[2] Fremeau, R. T., Jr., Kam, K., Qureshi, T., Johnson, J., Copenhagen, D. R., Storm-Mathisen, J., … Edwards, R. H. (2004). Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites. Science, 304(5678), 1815–1819.

[3] Choi, D. W. (1988). Glutamate neurotoxicity and diseases of the nervous system. Neuron, 1(8), 623–634. 

[4] Shi, Y., Kirwan, P., Smith, J., Robinson, H. P. C., & Livesey, F. J. (2012). Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses. Nature Neuroscience, 15(3), 477–486.