Recommended Medium: It is recommended to use Neuronal Maintenance Medium (NMM, Cat. no. SC5951) for culturing HPSC-CN in vitro.
Product Use: HPSC-CN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Douglas, R. J., & Martin, K. A. C. (2004). Neuronal circuits of the neocortex. Annual Review of Neuroscience, 27, 419–451.
[2] Hensch, T. K. (2005). Critical period plasticity in local cortical circuits. Nature Reviews Neuroscience, 6(11), 877–888.
[3] Shi, Y., Kirwan, P., Smith, J., Robinson, H. P. C., & Livesey, F. J. (2012). Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses. Nature Neuroscience, 15(3), 477–486.
[4] Espuny-Camacho, I., Michelsen, K. A., Gall, D., Linaro, D., Hasche, A., Bonnefont, J., … Vanderhaeghen, P. (2013). Pyramidal neurons derived from human pluripotent stem cells integrate efficiently into mouse brain circuits in vivo. Neuron, 77(3), 440–456.