Cell Source
Frozen human hepatocytes offered by 3H Biomedical are isolated from human liver by Biopredic International according to the method of Guguen-Guillouzo C and Guillouzo A (In “Isolated and cultured hepatocytes”, Guillouzo A. and Guguen-Guillouzo C, Eds. INSERM Paris & John Libbey Eurotext London, 1986, pp.1-12.).
Cell Characterization
Frozen human hepatocytes are negative for HIV 1-2, HBV, HCV, mycoplasma, bacteria, yeast and fungi. The cells are genotyped for CYP2C9, CYP2C19, CYP2D6 and CYP3A5, and are characterized by the activities of CYP1A2, CYP2C9, CYP3A4/5, Paracetamol glucuronidation and Paracetamol sulfation.
Cell Culture Condition
The cells are cryopreserved immediately after purification and are transported at -135°C. If storage of the cells is desired, liquid nitrogen is recommended. Culture the frozen human hepatocytes in a monolayer or in suspension at the conditions provided in the Instruction for use, and use the cells 24 hours after seeding.
Recommended Cell Culture Medium
• Hepatocyte thawing medium without glucose (100 ml, Cat. no. BMIL261)
• Williams medium for hepatocyte seeding (100 ml, Cat. no. BMIL212)
• Williams medium for hepatocyte incubation (100 ml, Cat. no. BMIL214-100ML)
• Minimum Essential Medium (MEM) Earle – without phenol red (100o ml, Cat. no. BMIL236)
Cell Culture Reagents
• Percoll solution (100 ml, Cat. no. BPER212)
• Trypan blue solution (0.05% w/v, 100 ml, Cat. no. SR1007)
Shipping
• Liquid nitrogen vapor shipper, non-hazardous container
• Dry-ice (only within 24 hours upon on customer request)
Storage
• Liquid nitrogen
The product is for laboratory research use only. Not approved for human use.