Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete extracellular matrix which is rich in type I and/or type III collagen. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion, which plays a fundamental role in regulating immune response and homeostasis of tissue fluids.
Human Lymphatic Fibroblasts (HLF) are isolated from human lymph node. HLF are cryopreserved at P0 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HLF are characterized by their spindle-shaped morphology and immunofluorescence with antibody specific to fibronectin.
HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HLF are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for culturing HLF in vitro.
Product Use
HLF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.