HMMEC are isolated from human breast. HMMEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM), and by uptake of DiI-Ac-LDL. HMMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMMEC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Endothelial Cell Medium (ECM, Cat. No. SC1001) for the culturing of HMMEC.
Product Use: HMMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping: Dry ice.
Reference: [1] KM. Ahem, MR. Davis & IM. Sordillo (1995) Isolation and characterization of bovine mammary endothelial cells. Method in Cell Science. 17(1):41-46.
[2] AG. Naccarato, P. Viacava, G. Bocci, G. Fanelli, P. Aretini, A. Lonobile, G. Montruccoli and G. Bevilacqua (2003) Definition of the microvascular pattern of the normal human adult mammary gland. J. Anat. 203:599-603.
[3] V. Sigurdsson, AJR. Fridriksdottir. J. Kjartansson, JG. Jonasson, M. Steinarsdottir, OW. Petersen, HM. Ogmundsdottir, and T Gudjsonsson (2006) Human breast microvascular endothelial cells retain phenotypic traits in long-ter finite life span culture. In Vitro Cellular & Developmental Biology - Animal 42(10):332-340.