Human Stellate Cell Medium is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human hepatic stellate cells in vitro.
Components
SteCM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. SC0010), 5 ml of stellate cell growth supplement (SteCGS, Cat. No. SC5352) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
Product Use
SteCM is for researchuse only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at +4°C, the SteCGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping
Dry ice.