Cell line, continuous culture
Immortalization Method: N/A
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Kidney
Organ Type: Kidney
Growth Properties: Adherent
Morphology: Polyglonal
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. 3:1 MEM (11095098 Fisher):F12K (ATCC® 30-2004 Fisher) + 10% FBS + 8 mM D-glucose (15023021 Thermo Fisher) + 10 ng/ml human EGF (Z100139) + 2 mM L-glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Heilig, C. W., Concepcion, L. A., Riser, B. L., Freytag, S. O., Zhu, M., & Cortes, P. (1995). Overexpression of glucose transporters in rat mesangial cells cultured in a normal glucose milieu mimics the diabetic phenotype. The Journal of clinical investigation, 96(4), 1802–1814. https://doi.org/10.1172/JCI118226Dumler, F., & Cortes, P. (1988). Uracil ribonucleotide metabolism in rat and human glomerular epithelial and mesangial cells. The American journal of physiology, 255(6 Pt 1), C712–C718. https://doi.org/10.1152/ajpcell.1988.255.6.C712Wang, Y., Heilig, K. O., Minto, A. W., Chen, S., Xiang, M., Dean, D. A., Geiger, R. C., Chang, A., Pravtcheva, D. D., Schlimme, M., Deb, D. K., Wang, Y., & Heilig, C. W. (2010). Nephron-deficient Fvb mice develop rapidly progressive renal failure and heavy albuminuria involving excess glomerular GLUT1 and VEGF. Laboratory investigation; a journal of technical methods and pathology, 90(1), 83–97. https://doi.org/10.1038/labinvest.2009.95