Immortalized Sweat Gland Myoepithelial Cells provide a useful
in vitro model for recapitulating accrine sweat gland functions and for screening substances that can modulate sweating by acting on sweat glands. These cells are applicable for studies in hyperhidrosis, adiaphoresis, and sweat gland diseases.
Immortalization Method: Immortalized with lentiviral vector containing hTERT & SV40Tt
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Eccrine Sweat Gland
Organ Type: Eccrine Sweat Gland
Growth Properties: Suspension
Morphology: Spheroid
Population Doubling: 24 - 46 hours
Markers: Puromycin, Alpha-SMA
Donor Age: 53
Donor Gender: Female
Propagation: Ultra-Low Attachment Plates are required for cell growth.The base medium for this cell line is Mammocult Human Medium Kit (STEMCELL Technologies). To make the complete growth medium, add the following components to the medium kit: Recombinant Human EGF (Z100135) to a final concentration of 10 ng/ml, Recombinant Human bFGF (Z101455) to a final concentration of 10 ng/mL, heparin to a final concentration of 4 µg/ml,hydrocortisone 21-hemisuccinate (Sigma) to a final concentration of 0.5 µg/ml, growth factor reduced matrigel (BD Biosciences) to a final concentration of 2% (v/v), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
Quality Control: 1) Immunofluorescence; 2) Fluorescence microscopy
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C