Immortalization Method: Immortalization with SnRV
BioSafety Level: II
Organism: Gilthead Sea Bream (S. aurata)
Species: Gilthead Sea Bream
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Epithelial
Markers: Glial and neuronal markers by IHC
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow XIII (TM013) + 10% FBS + 2 mM L-glutamine (G275) + 10 mM HEPES + 1% Penicillin/Streptomycin Solution (G255), +20-+25.0°C
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Cuesta, A., Ruiz-Palacios, M., Martins, M.A., et al. (2020). Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology, Science of The Total Environment, 708(15), 134821, https://doi.org/10.1016/j.scitotenv.2019.134821Ruiz-Palacios, M., Esteban, M.A., & Cuesta, A. (2020). Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology, Fish and Shellfish Immunology, 106, 161-166, https://doi.org/10.1016/j.fsi.2020.07.065