Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2)

Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) is pluripotent and has the appearance of both neuronal and glial morphologies in culture. The cells are able to differentiate into different phenotypes, where neuronal markers are expressed (NSE, PGP, and MAP-2), but glial markers are absent. It is a valuable tool for studies relating to the regulation of the central mammalian pacemaker function, and the development of the suprachiasmatic nucleus cell types.

Immortalization Method: Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene

BioSafety Level: II

Species: Rat

Source Organ: Brain

Growth Properties: Adherent

Morphology: Round|Flattened

Population Doubling: 17-27 hours

Seeding Density: 10,000 cells/cm²

Markers: NSE, PGP, and MAP-2

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: Grow cells in poly-D-lysine or laminin-coated plates. The base medium for this cell line is Prigrow VII, available at abm Cat. No. TM017. To make the completed growth medium, add the following components to the base medium for a final concentration of fetal bovine serum (TM999)* to a final concentration of 10%, 2 µg/ml glucose, 2 mM L-glutamine, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Immunofluorescent staining and western blot analysis for E1A; 2) Immunocytochemical screening with cell type-specific antigens to distinguish phenotypes

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C