Immunofluorescent staining and western blot analysis for E1A
Immortalization Method: Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene
BioSafety Level: II
Organism: Rat (R. norvegicus)
Species: Long-Evans Rat
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Flat rounded appearance
Donor Age: fetal
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 10 mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Earnest, D. J., Liang, F. Q., DiGiorgio, S., Gallagher, M., Harvey, B., Earnest, B., & Seigel, G. (1999). Establishment and characterization of adenoviral E1A immortalized cell lines derived from the rat suprachiasmatic nucleus. Journal of neurobiology, 39(1), 1–13. https://doi.org/10.1002/(sici)1097-4695(199904)39:1