Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor (GFSHR-17)

Follicle stimulating hormone (FSH) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately FSH receptor expression is often lost within 1-2 days of in vitro culture of primary cells.


The Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor is responsive to FSH stimulation and produces progesterone as a result at levels comparable to primary cells. This cell line is useful in studying FSH-FSH receptor interactions, as well as how FSH receptor regulates the development and maturation of ovarian follicle. Together with the Immortalized Rat Steroidogenic Granulosa Cells expressing LH Receptor (Cat. No
http://www.abmgood.com/Immortalized-Rat-Steroidogenic-Granulosa-Cells-stably-expressing-LH/CG-Receptor-T0606.html"> T0606), researchers can compare the LH and FSH signaling in homologues cell systems.

Immortalization Method: Serial passaging and calcium phosphate mediated cotransfection by SV40 DNA and Ha-ras oncogene

BioSafety Level: II

Species: Rat

Source Organ: Ovary

Growth Properties: Adherent

Morphology: Multi-Polar

Population Doubling: 35 - 45 hours

Seeding Density: 10,000 - 20,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.



To stimulate progesterone production, add 1.6 nM FSH, 100 nM dexamethasone and 50 µM forskolin to the complete culture medium for at least 24 hours. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Phenotypic response to FSH was characterized by shape changes (cells rounding up); 2) FSH receptor expression was measured by radiolabelled 125I-oFSH binding assay- the cells express 27,000 FSH receptors/cell with a Kd of 100-115 pM, similar to the native receptor; 3) Production of progesterone was determined by radioimmunoassay (RIA); 4) Production of cAMP was measured by a protein binding method

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C