Immortalized Rat Retinal Cells (E1A-NR.3)

Immortalized Rat Retinal Cells (E1A-NR.3) are immortalized with the 12S portion of replication incompetent adenovirus E1A gene. It expresses vimentin and is the parental cells to the Immortalized Rat Retinal Precursor Cell Line (R28), also available at abm (T0575). E1A-NR.3 cells exhibit contact inhibition and anchorage-dependent growth. Researchers may use this cell line to study photoreceptor rescue or replacement paradigm as studies have shown that the Immortalized Rat Retinal Cells (E1A-NR.3) are capable to successfully be transplanted intraocularly.

Immortalization Method: Serial passaging and transduction with retroviruses carrying 12S portion of replication incompetent adenovirus E1A gene

BioSafety Level: II

Species: Rat

Source Organ: Retina

Growth Properties: Adherent

Morphology: Neuronal

Population Doubling: Doubling time of 24 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
Split 1:2 when reach 80% confluency
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunoreactivity was assessed in vitro and in vivo for markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C