Immortalization Method: Serial passaging and transduction with replication incompetent recombinant lentiviruses carrying SV40 Large T antigen BioSafety Level: IISpecies: RatSource Organ: Rat brainGrowth Properties: AdherentMorphology: Stellate shapedPopulation Doubling: 24-34 hoursSeeding Density: 30,000 cells/cm²; Recommended split ratio is 1:2Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 4.5g/L D- Glucose (in addition to 4.5g/L D-glucose already present in Prigrow III) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C