Immortalized Rat Luteal Cells-Conditionally (GG-CL)

The corpus luteum develops from an ovarian follicle during the luteal phase of the menstrual cycle and is responsible for the decidualization of the endometrium. The Immortalized Rat Luteal Cells (GG-CL) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and has retained many primary luteal cell markers such as estrogen receptor-β, glucocorticoid receptor (GR), interleukin-6 (IL-6), orphan nuclear receptor nur 77 and 20α-hydroxysteroid dehydrogenase (20α-HSD). In addition, GG-CL responds to stimulation by PRL, estradiol, and cAMP, making the cells a useful model for studies involving gene expression and regulation of luteal cells.

Immortalization Method: Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus

BioSafety Level: II

Species: Rat

Source Organ: Corpora lutea

Growth Properties: Adherent

Morphology: Flattened

Population Doubling: 17 - 27 hours

Seeding Density: 30,000 - 40,000 cells/cm²

Markers: IL-6, ER-β, GR, 20α-HSD, JAK2

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/streptomycin to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C. The functional expression of the SV40 antigen is induced by culturing the cells in vitro at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and revert to differentiated phenotype.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Northern analysis and RT-PCR were used to confirm the expression of IL-6, glucocorticoid receptor (GR), estrogen receptor-β (ER-β) and orphan nuclear receptor nur 77. 2) Cells were subjected to treatments of exogenous prolactin, estradiol, and cAMP to examine effects.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C