Immortalized Rat Embryonic Striatal Wildtype (121-1I) Cells

Immortalized Rat Embryonic Striatal Wildtype (121-1I) Cells are immortalized with the the A58 temperature-sensitive allele of the SV40 large T antigen. It is a non-GABAergic cell line, in which there is no GAD expression. It may be used as a wild type to compare to the GABAergic cell lines such as Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T (T0356), Immortalized Rat Embryonic Striatal (M26-1F) Cells - tsSV40T (T0357), or Immortalized Rat Embryonic Striatal (M213-1L) Cells (T0757).

Immortalization Method: Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen

BioSafety Level: II

Species: Rat

Source Organ: Straitum

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 42 - 52 hours

Seeding Density: 20,000 - 30,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C