Immortalized Rat Embryonic Striatal Wildtype (121-1I) Cells are immortalized with the the A58 temperature-sensitive allele of the SV40 large T antigen. It is a non-GABAergic cell line, in which there is no GAD expression. It may be used as a wild type to compare to the GABAergic cell lines such as Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T (T0356), Immortalized Rat Embryonic Striatal (M26-1F) Cells - tsSV40T (T0357), or Immortalized Rat Embryonic Striatal (M213-1L) Cells (T0757).
Immortalization Method: Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen
BioSafety Level: II
Species: Rat
Source Organ: Straitum
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 42-52 hours
Seeding Density: 20,000-30,000 cells/cm²
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: N/A
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C