Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T has GABAgeric properties with multiple nucleoli, and no contact inhibition features. It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (+33°C) in which SV40 expression is observed. In non-permissive temperature (+39.5°C), the morphology of the cells will become elongated and no longer expresses SV40. The growth becomes slower but the cells express GFAP.
Immortalization Method: Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen
BioSafety Level: II
Species: Rat
Source Organ: Straitum
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: N/A
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: +33.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Immunostaining for SV40 T large antigen and markers; 2) Test growth in permissive and non-permissive temperatures
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C