Immortalized Rat Embryonic Striatal (M213-1L) Cells

Immortalized Rat Embryonic Striatal (M213-1L) Cells is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington's disease.

Immortalization Method: Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen

BioSafety Level: II

Species: Rat

Source Organ: Striatum

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 20 - 30 hours

Seeding Density: 20,000 - 30,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C