Immortalized Rat Decidual Cells-Conditionally (GG-AD)

The decidual cells play an important role in the maintenance of pregnancy at the maternofetal interface after implantation of the fertilized egg into the endometrium. The Immortalized Rat Decidual Cells (GG-AD) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and possesses several endocrinological features, including the expression of progesterone receptor, activin type II, activin βA and activin βB. In addition, it also retains the ability to respond to cAMP stimulation and secrete PRL-related hormones, a hallmark of the antimesometrial decidual cells. This cell line is useful for studying the mechanism of decidual cell regression and reorganization.

Immortalization Method: Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus

BioSafety Level: II

Species: Rat

Source Organ: antimesometrial decidua

Growth Properties: Adherent

Morphology: Binucleated|Compact|Polynucleated|Flattened

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: IL-6, IL-6R, gp130, activin βA, decidual PRL-related protein (dPRP)

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/streptomycin to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C. The functional expression of the SV40 antigen is induced by culturing the cells in vitro at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and revert to differentiated phenotype.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Northern blot performed to analyze presence of activin βA , follistatin and PLP-B mRNA; 2) RT-PCR analysis of progesterone receptor, activin βA, activin βB, and activin receptor II mRNA was completed with L19 ribosomal protein mRNA as control.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C