Immortalized Rat Cochlear Microglia Cells (Mocha)

Immortalized Rat Cochlear Microglia Cells (Mocha) are derived from Sprague-Dawley rat cochlea. Mocha cells express neomycin resistance gene, but do not need to be cultured in it anymore. G418 was used for selection to ensure homogenous population. It has been reported that the Mocha cells respond well to lipopolysaccharide treatments, and phagocytose fluorescent beads. Immortalized Rat Cochlear Microglia Cells (Mocha) are a unique cell line ready for research use.

Immortalization Method: Immortalized with retrovirus containing 12S-E1A gene

BioSafety Level: II

Species: Rat

Source Organ: Cochlea

Growth Properties: Adherent

Morphology: Flat, with occasional processes

Population Doubling: Approximately 24 hours

Seeding Density: N/A

Markers: Neomycin resistance, TMEM119, CD45, Iba1, CD68, Il1-beta

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal calf serum (Hyclone) to a final concentration of 5%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) PCR gene array; 2) ELISA; 3) ICC

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C