Immortalized Rat Cardiomyocytes

Immortalization Method: Serial passaging and transduction with recombinant lentivector carrying SV40 Large T antigen

BioSafety Level: II

Species: Rat

Source Organ: Heart/neonatal

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 48-60 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: 1-2 days

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 15% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Western blot was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C