Immortalization Method: Serial passaging and transduction with recombinant lentivector carrying SV40 Large T antigen
BioSafety Level: II
Species: Rat
Source Organ: Heart/neonatal
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 48-60 hours
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: 1-2 days
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No.
TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to a final concentration of 15% and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Western blot was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C