Immortalization Method: Serial passaging and infection with lentivirus carrying SV40 gene
BioSafety Level: II
Organism: Rat (R. norvegicus)
Species: Rat
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Endothelial
Population Doubling: 24 - 36 hours
Seeding Density: 30,000 cells/cm^²
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C