Subpopulations of adipocytes have distinct cellular responses and metabolism and can be used to study behavioural and physiological differences in fat depots.
Immortalization Method: Conditionally immortalized. Cells were derived from the Immortomouse and grown at +33°C with 10 units/ml interferon-gamma.
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Adipose
Organ Type: Adipose
Growth Properties: Adherent
Morphology: Fibroblast-like
Population Doubling: 24-48 hours
Seeding Density: 20,000-25,000 cells/cm2
Markers: Characterization markers (qPCR): Mx1, Casp1, Cxcl12
Donor Gender: Male
Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, GlutaMAX (Gibco) to a final concentration of 2mM, recombinant mouse IFNG (Z200085) to a final concentration of 10 U/ml and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +33.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) qPCR; 2) Oil RedO staining
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Lee, K. Y., Sharma, R., Gase, G., Ussar, S., Li, Y., Welch, L., Berryman, D. E., Kispert, A., Bluher, M., & Kahn, C. R. (2017). Tbx15 Defines a Glycolytic Subpopulation and White Adipocyte Heterogeneity. Diabetes, 66(11), 2822–2829. https://doi.org/10.2337/db17-0218Lee, K. Y., Luong, Q., Sharma, R., Dreyfuss, J. M., Ussar, S., & Kahn, C. R. (2019). Developmental and functional heterogeneity of white adipocytes within a single fat depot. The EMBO journal, 38(3), e99291. https://doi.org/10.15252/embj.201899291