Immortalization Method: Transfecting human telomerase reverse transcriptase (hTERT)
BioSafety Level: II
Organism: Pig (Porcine)
Species: Pig (Porcine)
Source Organs: Pancreas
Organ Type: Pancreas
Growth Properties: Adherent
Morphology: spindle-shaped and fibroblast-like
Population Doubling: 22 hours
Seeding Density: 35,000 - 40,000 cells/cm
2Markers: Vim, PC1/3, Glut2, PDX1 (detected by IF), Ngn3, Mafa, NeuroD1 (detected by RT-PCR), G418 resistance (400 µg/ml)
Donor Age: 2 months old
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow VI (TM016) + 15% FBS + 2 mM L-glutamine (G275) + 0.1 mM β-mercaptoethanol + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C