Immortalization Method: N/A
BioSafety Level: II
Organism: Pig (Porcine)
Species: Pig (Porcine)
Source Organs: Kidney
Organ Type: Kidney
Growth Properties: Adherent
Morphology: Fibroblast-like
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Mays, C. E., Kang, H.-E., Kim, Y., Shim, S. H., Bang, J.-E., Woo, H.-J., … Ryou, C. (2008). CRBL cells: Establishment, characterization and susceptibility to prion infection. Brain Research, 1208, 170–180. https://doi.org/10.1016/j.brainres.2008.02.103He, S., Li, Y., Chen, Y., Zhu, Y., Zhang, X., Xia, X., & Sun, H. (2016). Immortalization of pig fibroblast cells by transposon-mediated ectopic expression of porcine telomerase reverse transcriptase. Cytotechnology, 68(4), 1435–1445. https://doi.org/10.1007/s10616-015-9903-8