Immortalized Porcine Aortic Endothelial Cells (AOC)

Recently proposed as a potential model of atherosclerosis, allograft rejection and accommodation, xenotransplantation and viral haemorrhage diseases, the porcine endothelial cells provide a useful tool in studies including the molecular mechanisms of xenogeneic rejection in the swine-to-human combination, and the pathogenesis of African Swine Fever (ASF) and Classical Swine Fever (CSF). The Immortalized Porcine Aortic Endothelial Cells (AOC) is derived from stable transformation of SV40 genome into primary porcine aortic endothelial cells. The resulting AOC retains comparable surface markers to its primary cell counterparts, as well as preserving its ability to uptake acetylated low density lipoproteins (Ac-LDL) and its susceptibility to xenogeneic cell-mediated cytotoxicity (i.e. Human Natural Killer Cell-mediated lysis).

Immortalization Method: Tranfection with pRNS-1 plasmid encoding the SV40 genome

BioSafety Level: II

Species: Pig

Source Organ: Aorta

Growth Properties: Adherent

Morphology: Cobble-stone

Population Doubling: 50-60 hours

Seeding Density: 25,000-30,000 cells/cm²; Recommended split ratio is 1:2 to 1:3

Markers: CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 20% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens; 2) ⁵¹Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity; 3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI)-labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C