Immortalized Porcine Alveolar Macrophage Cells (PAM-KNU) - hTERT

Immortalized Porcine Alveolar Macrophage Cells (PAM-KNU) - hTERT are derived from the lung of a euthanized 8-week-old SPF pig. Immortal cells were selected in the presence of 1 mg/ml G418. Suitable for research areas related to virus pathogenesis and host cell interactions.

Immortalization Method: Immortalized with retrovirus containing hTERT (pFB-Neo-hTERT)

BioSafety Level: II

Species: Porcine

Source Organ: Lung

Growth Properties: Adherent

Morphology: Macrophage-like

Population Doubling: 24-36 hours

Seeding Density: N/A

Markers: CD163

Donor Age: 8 weeks

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, 20 µl/ml Dexamethasone (Sigma), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise. Recommended Split Ratio: 1:5.

Quality Control: 1. hTERT was determined via RT-PCR.
2. Presence of CD163 detected by FACS.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C