Immortalization Method: Immortalized with a modified SV40 large T vector
BioSafety Level: II
Organism: Pig (Porcine)
Species: Pig (Porcine)
Source Organs: Bone Marrow
Organ Type: Bone Marrow
Growth Properties: Adherent
Morphology: Endothelial
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 10 mM HEPES + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂. Selection with 0.2 mg/ml G418 (G271)
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Seebach, J. D., Schneider, M. K. J., Comrack, C. A., LeGuern, A., Kolb, S. A., Knolle, P. A., Germana, S., DerSimonian, H., LeGuern, C., & Sachs, D. H. (2001). Immortalized bone-marrow derived pig endothelial cells. Xenotransplantation (Københaven), 8(1), 48-61. https://doi.org/10.1034/j.1399-3089.2001.00075.x