Immortalization Method: Spontaneous immortalization
BioSafety Level: II
Organism: Rat (R. norvegicus)
Species: Rat
Source Organs: Liver
Organ Type: Liver
Growth Properties: Adherent
Morphology: Epithelial
Population Doubling: 28 hours
Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow I (TM001) + 10% FBS + 2 mM L-glutamine (G275) + 1X NEAA + 1X Chemically Defined Lipid Concentrate + 1X MEM Vitamin Solution + 1X ITS + 50 µg/ml soybean trypsin inhibitor + 15 µg/ml BPE + 25 ng/ml EGF + 393 ng/ml dexamethasone + 3.4 µg/ml T3
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Muff, M. A., Masyuk, T. V., Stroope, A. J., Huang, B. Q., Splinter, P. L., Lee, S. O., & Larusso, N. F. (2006). Development and characterization of a cholangiocyte cell line from the PCK rat, an animal model of Autosomal Recessive Polycystic Kidney Disease. Laboratory investigation; a journal of technical methods and pathology, 86(9), 940–950. https://doi.org/10.1038/labinvest.3700448