Immortalized Ovarian Surface Epithelial Cells (iMOSEC)

The Immortalized Ovarian Surface Epithelial Cells (iMOSEC) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. This cell line is a useful model for studying ovary growth regulation.

Immortalization Method: Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD

BioSafety Level: II

Species: Mouse

Source Organ: Ovarian surface

Growth Properties: Adherent

Morphology: Cuboidal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: E-cadherin, cytokeratin 8 and cytokeratin 18.

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C