Immortalized Normal Human Urothelial Cells (NHU) - hTERT were immortalized via retroviral transduction (pLXSN-hTERT). Immortalized cells were selected in the presence of G418 (selected using 0.1 mg/mL).
Immortalization Method: Immortalized with retrovirus containing hTERT (pLXSN-hTERT)
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Ureter
Organ Type: Ureter
Growth Properties: Adherent
Morphology: Epithelial
Population Doubling: 16 - 20 hours
Markers: Neomycin resistance G418 (selected using 0.1 mg/mL)
Donor Age: 63
Donor Gender: Female
Propagation: The base medium for this cell line is Prigrow X medium available at
abm, Cat. No. TM0785.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
Quality Control: 1) Immunocytochemistry; 2) CGH
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Georgopoulos, N. T., Kirkwood, L. A., Varley, C. L., MacLaine, N. J., Aziz, N., & Southgate, J. (2011). Immortalisation of normal human urothelial cells compromises differentiation capacity. European urology, 60(1), 141–149. https://doi.org/10.1016/j.eururo.2011.02.022