Immortalized Normal Human Oral Mucosal Keratinocytes

Immortalized Normal Human Oral Mucosal Keratinocytes (OMK) were isolated from human buccal mucosa. This cell line was immortalized using a vector carrying HPV16 E6/E7 genes. Cell lysates of immortalized OMKs are reliable substrates for the detection of mucous membrane pemphigoid autoantigens.

Immortalization Method: Immortalized with lentiviral vector carrying HPV16 E6/E7 genes (pLVSIN-EF1-HPV16-E6_7-Pur)

BioSafety Level: II

Species: Human

Source Organ: Oral mucosa

Growth Properties: Adherent

Morphology: Epithelial-like

Population Doubling: 24 - 34 hours

Seeding Density: N/A

Markers: N/A

Donor Age: 34

Donor Gender: Female

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0768.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C