Description: Immortalized Multimammate Mouse Skin Keratinocytes (Kera5) are a spontaneously immortalized cell line derived from primary keratinocytes of newborn virus-free Mastomys coucha. Mastomys coucha-derived skin keratinocytes can be used as an in vitro system to investigate molecular and immunological aspects of infectious agent interactions with their host cells.Immortalization Method: SpontaneousBioSafety Level: IISpecies: MouseSource Organ: SkinGrowth Properties: AdherentMorphology: Cobble-stonePopulation Doubling: 24 - 34 hoursSeeding Density: N/AMarkers: Keratin-14Donor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0763.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: 1) ImmunofluorescenceShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C