Originally isolated from human umbilical cord blood, and are non-hematopoietic stem cells with many characteristics in common with mesenchymal stem cells
Immortalization Method: Transfected with the gene for TERT, cloned to pure populations by limited dilution culture and were expanded
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Cord Blood
Organ Type: Cord Blood
Growth Properties: Adherent
Morphology: Spindle shaped|Fibroblast-like
Population Doubling: 24 - 28 hours
Seeding Density: 10,000-20,000 cells/cm^2
Donor Gender: Male
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Undifferentiated Multi-Lineage Progenitor Cell Media Kit (TM113), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Collins, D. P., Hapke, J. H., Aravalli, R. N., & Steer, C. J. (2020). Development of immortalized human hepatocyte-like hybrid cells by fusion of multi-lineage progenitor cells with primary hepatocytes. PloS one, 15(6), e0234002. https://doi.org/10.1371/journal.pone.0234002
Collins, D. P., Hapke, J. H., Aravalli, R. N., & Steer, C. J. (2020).
in vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells. Hepatic medicine : evidence and research, 12, 79–92. https://doi.org/10.2147/HMER.S245916
Collins, D. P., & Steer, C. J. (2021). Binding of the SARS-CoV-2 Spike Protein to the Asialoglycoprotein Receptor on Human Primary Hepatocytes and Immortalized Hepatocyte-Like Cells by Confocal Analysis. Hepatic medicine : evidence and research, 13, 37–44. https://doi.org/10.2147/HMER.S301979