Immortalized Mouse Spleen Dendritic Cells (SRDC)

The Immortalized Mouse Spleen Dendritic Cell (SRDC) line is functionally and phenotypically similar to dendritic cells; specifically, in its antigen presentation, T cell priming, and dendritic cell marker expression. This innovative CD4
^-
CD205
⁺
CD11b
^- immortalized dendritic cell line eliminates the need for in vitro differentiation and expansion of different dendritic cell precursors into splenic dendritic cells. Therefore, these readily cultured cells are ideal for immunological studies focused on the function and biology of dendritic cells.

Immortalization Method: Serial passaging and liposome-mediated transfection with pSV3neo plasmid carrying the SV40 T oncogene

BioSafety Level: II

Species: Mouse

Source Organ: Spleen

Growth Properties: Adherent

Morphology: Dendritic-like

Population Doubling: 20-24 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: HuD, PGP9.5, tau, MHC-I, MHC-II, CD80, CD40, CD11c, CD59, CD205

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow V medium available at abm (TM015). To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999)* to final concentration of 5%, 2mM L-glutamine, 50 µM ?-Mercaptoethanol and Penicillin/Streptomycin Solution (G255) to final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1. Immunofluorescence microscopy analysis confirmed SRDC maturation- through expression of DC-LAMP and MHC-II expression.
2. ELISA assays to determine interleukin and gamma interferon production.
3. To confirm dendritic cell transformation, SV40 large T antigen expression was compared between SRDCs and Cos-7-positive control cells and BHK-21-negative control cells.
4. PCR analysis confirmed that the SV40 oncogene was not integrated into SRDC cellular DNA.
5. Antigen uptake ability tested via incubation with antitoxoplasma serum.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C