Immortalized Mouse Sertoli Cells (D-4-A) are derived from transgenic mice expressing polyomavirus large T antigen. These cells are immortalized and are not capable of forming tumors. Morphology exhibited by the cells is large cells with extensive cytoplasmic elongations in appearance, but changes at higher densities exhibiting a cuboidal appearance. The Immortalized Mouse Sertoli Cells (D-4-A) are a reliable tool to use to investigate sertoli biology.
Immortalization Method: Derived from transgenic mice expressing polyomavirus large T antigen
BioSafety Level: II
Species: Mouse
Source Organ: Testicle
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: Transferrin
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) 50 µg/ml Gentamycin used for selection. 2) RT-PCR used to detect Sertoli cell markers
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C