Immortalized Mouse Retinal Cells (MU-PH1)

Immortalized Mouse Retinal Cells (MU-PH1) are Müller-derived retinal progenitor cells which retained characteristics of photoreceptor, glial and stem cells. MU-PH1 cells are light sensitive and have expression of the TLR1, TLR2, TLR4, TLR6, MT1 and MT2 receptors. Additionally it had been found to express photoreceptor markers such as rhodopsin, recoverin, transducin, blue opsin and red/green opsin. The cells were spontaneously immortalized in culture and may be utilized for studies relating to retinal stem cell biology.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Retina

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: Vimentin, S-100, glutamine synthetase, nestin, ABCG2,a-tubulin,b-III-tubulin and Ascl1

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: RT-PCR, immunofluorescence and Western blot were used to identify cell markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C