Immortalized Mouse Proximal Tubule KPT11 Cells

Immortalized Mouse Proximal Tubule KPT11 Cells are spontaneously immortal derived cells from murine kidney. KPT11 cells are more susceptible to gentamicin treatments, as gentamicin-induced toxicity was observed compared to the Immortalized Mouse Distal Tubule KDT3 Cells (abm, T0641). It is found that the KPT11 cells have a higher density of gentamicin-binding proteins, such as CLIMP-63. This cell line is a valuable tool for studies relating to the mechanisms of the gentamicin-binding proteins and effects of gentamicin in apoptosis.

Immortalization Method: Spontaneously immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Kidney

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: 20,000-50,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: RT-PCR and Western blot were used to detect the presence of CLIMP-63

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C