The Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11) was isolated from a subpopulation of cells in the heterogeneous COMMA-1D mouse mammary epithelial cell. The subpopulation was isolated based on its 4-fold higher β-casein milk protein production after lactogenic hormone induction with dexamethasone, insulin and prolactin in comparison to parental population of COMMA-1D cells. The Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells are applicable in studies related to prolactin-regulated genes.
Immortalization Method: Transfection with pY3 plasmid
BioSafety Level: II
Species: Mouse
Source Organ: Mammary glands
Growth Properties: Adherent
Morphology: Cuboidal
Population Doubling: N/A
Seeding Density: Recommended split ratio of 1:5
Markers: Hygromycin B resistance
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to final concentration of 10%, 50 µg/ml gentamicin (G272), 5 µg/ml insulin (TM053), 10 ng/ml epidermal growth factor (Z200025), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: 1) Immunofluorescence using anti-casein antibody; 2) Southern blot for β-casein
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C