Immortalized Mouse Preadipocyte Cells (ScAP-23)

The Immortalized Mouse Preadipocyte Cells (ScAP-23) was isolated from subcutaneous adipose tissue from C57BL/6 mice and immortalized by infection with retroviruses carrying hTERT gene. The ScAP-23 preadipocytes convert to adipocytes when treated with dexamethasone, 3-methylisobutylxanthine, insulin, and indomethacin. Expression of key adipogenic transcription factors such as PPARɣ, C/EBP family, SREBP-1c transcription factors and Krox20/Egr2 are found upregulated during ScAP-23 adipogenesis. The ScAP-23 adipocytes contain abundant lipid droplets and express transcripts for mature adipocytes markers such as SCD1, aFABP, ATGL, FAS, LDL, and GPDH. Furthermore, ScAP-23 adipocytes demonstrate insulin responsiveness and express insulin receptor-β and major insulin-responsive glucose transporter GLUT4. Thus, the cell line is recommended as a tool for adipocytes biology and adipogenesis research.

Immortalization Method: Serial passaging and infection with retroviruses carrying hTERT gene

BioSafety Level: II

Species: Mouse

Source Organ: Adipose tissue

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium (TM003). To make the completed growth medium, add the following components to the base medium at a final concentration of fetal bovine serum (TM999)* to a final concentration of 10%, 2mM L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Oil Red O staining at day 7 after inducing adipocyte differentiation to visualize lipid droplets; 2) Harvest RNA after induction of adipogenesis and perform RT-PCR and Northern Blot to detect transcript level of adipocyte markers SCD1, aFABP, ATGL, FAS, LDL, and GPDH B.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C