Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)

Description: IMPE is a conditionally immortalized mouse pancreas epithelial cell line isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2K^b promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C) or when cultured without IFN γ over 3 passages, the cells cease to proliferate. The IMPE has been shown to produce insulin after transfection with pdx1 and culture in the presence of glucagon-like peptide 1 (GLP1).

Immortalization Method: Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)

BioSafety Level: II

Species: Mouse

Source Organ: Pancreas

Growth Properties: Adherent

Morphology: Cobble-stone

Population Doubling: N/A

Seeding Density: Recommended split ratio of 1:3

Markers: β-catenin, E-Cadherin, CK8, CK18, CK19

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, 5 U/ml murine IFN-γ and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Western Blot and immunofluorescence were used to detect the presence of SV40 large T antigen; 2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin; 3) RT-PCR was used to verify the expression of CK8, CK18 and CK19.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C