Immortalization Method: Spontaneously immortalized
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: 129Sv mice
Source Organs: Oral
Organ Type: Oral
Growth Properties: Adherent
Morphology: Epithelial
Donor Age: Six-month old
Donor Gender: Female
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series (TM0855) + 15% chelexed FBS + 26.8 mM sodium bicarbonate + 50 pM tri-iodothyronine + 127 pM cholera toxin + 1.9 pg/ml hydrocortisone + 12.7 µg/ml insulin + 12.7 µg/ml transferrin+ 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Parikh N, Nagarajan P, Sei-ichi M, Sinha S, Garrett-Sinha LA. Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin. Arch Oral Biol. 2008 Nov;53(11):1091-100. doi: 10.1016/j.archoralbio.2008.07.002. Epub 2008 Aug 21. PMID: 18721915; PMCID: PMC2573465.