Immortalization Method: Derived from H-2kb-tsA58 transgenic mouse
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: H-2kb-tsA58 transgenic mouse
Source Organs: Eye
Organ Type: Eye
Growth Properties: Adherent
Morphology: Polygonal
Seeding Density: 20,000 cells/cm²
Markers: GFAP and NCAM (detected by ICC)
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0845), +33.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Liu, Y., Patel, G. C., Mao, W., & Clark, A. F. (2018). Establishment of a conditionally immortalized mouse optic nerve astrocyte line. Experimental eye research, 176, 188–195. https://doi.org/10.1016/j.exer.2018.07.011