Immortalized Mouse Olfactory Bulb Cells (OBL24)

The olfactory bulb is located within the vertebrate forebrain, in which it functions to receive nerve endings/input from the nasal cavity. The sense of smell is associated with the nerve tissues of the olfactory bulb.
Immortalized Mouse Olfactory Bulb Cells (OBL24) is immortalized with non-replicating retrovirus carrying the avian Myc gene. They express neuronal markers such as neurofilament (NF), tetanus toxin (TT), GFAP, A2B5, and HNK-1. The OBL24 cells are found to be non-tumorigenic and could incorporate BrdU. It is a valuable in vitro tool for studies relating to the functions and the biology of the olfactory bulb.

Immortalization Method: Infection with retrovirus containing the avian Myc gene (pneoMLV)

BioSafety Level: II

Species: Mouse

Source Organ: Olfactory bulbs

Growth Properties: Adherent

Morphology: Flattened|Dendritic processes

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: Grow cells in Poly-L-Lysine (TM061)-coated flasks with the following conditions. The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C